Lateral flow colloidal gold-based lateral flow immunoassays is a convenient, sensitive and low-cost test method. However, electronic devices are needed for accurate quantification of the result, and the naked-eye observation can only provides very limited information related to the relative concentration comparison. Since this method is always performed in point-of-care, professional devices are not available and also cost too much time, we think it is useful to provide a system for the user to make a preliminary quantitative test of the samples.
Here we CDIA test provide a new system for semi-quantitative measure of the analytes. With series of optimization, the lines on the strip can mark for different cut-off values, thus users can distinguish the low, moderate and high concentration with a valid standard. And with more lines added, this method should be able to distinguish the concentration in a broader range.
Fang C, Chen Z, Li L, et al. Barcode lateral flow immunochromatographic strip for prostate acid phosphatase determination[J]. Journal of pharmaceutical and biomedical analysis, 2011, 56(5): 1035-1040.