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A Naked-Eye Semi-Quantitative LFI System

Lateral flow colloidal gold-based lateral flow immunoassays is a convenient, sensitive and low-cost test method. However, electronic devices are needed for accurate quantification of the result, and the naked-eye observation can only provides very limited information related to the relative concentration comparison. Since this method is always performed in point-of-care, professional devices are not available and also cost too much time, we think it is useful to provide a system for the user to make a preliminary quantitative test of the samples.

Here we CDIA test provide a new system for semi-quantitative measure of the analytes. With series of optimization, the lines on the strip can mark for different cut-off values, thus users can distinguish the low, moderate and high concentration with a valid standard. And with more lines added, this method should be able to distinguish the concentration in a broader range.


If you have any ideas or questions related to this semi-quantitative system, please feel free to contact us, we would be honored to communicate with you on this technique.


Fang C, Chen Z, Li L, et al. Barcode lateral flow immunochromatographic strip for prostate acid phosphatase determination[J]. Journal of pharmaceutical and biomedical analysis, 2011, 56(5): 1035-1040.

Yin H Y, Chu P T, Tsai W C, et al. Development of a barcode-style lateral flow immunoassay for the rapid semi-quantification of gliadin in foods[J]. Food chemistry, 2016, 192: 934-942.

Related reading: Immunochromatographic Assays Lateral Flow Tests

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