The CDIA™ Rapid Human Antibody Isotyping Kit determines the immunoglobulin class, subclass, and light chain type in a rapid 5-minute dipstick assay.
The CDIA™ Rapid Human Antibody Isotyping assay is a lateral flow screening test. In the assay, the properly diluted sample is allowed to run on a series of capillary beds. Following its application to the sample pad, the sample is pretreated and discharged onto the conjugate pad. Human Antibody Isotype Conjugate is released upon contact with the flowing sample and forms soluble complexes with the analytes. These complexes travel the length of the membrane and are captured in the test region by anti-isotype antibody immobilized on the nitrocellulose membrane. A red colored line formed in the test region indicates the presence and class, subclass, or light chain type of human antibodies in the test specimen. Absence of the colored line in the test region indicates a negative result. A red colored line in the control region (C) will always appear and indicates a properly functioning test.
The CDIA™ Rapid Human Antibody Isotyping Kit makes it quick and easy to determine human antibody class, subclass and light chain type.Determining the class and subclass of a human antibody is important in a variety of research areas including infectious disease, cancer, autoimmune diseases, immunodeficiency disorders, and drug and vaccine development. Isotyping is also important when developing and purifying humanized antibodies.