This kit is the quantitative detection of content of 25-hydroxyvitamin D (25-OH-Vit-D) in human serum samples.
Based on the principle of competitive method, the content of 25-OH-Vit-D in samples will be quantitatively detected by immunochromatography technology. The 25-OH-Vit-D in sample and 25 hydroxyvitamin-D-BSA protein compounds compete the binding site of the fluorescent marked rat- anti-human 25-OH-Vit-D antibody on the microspheres. After a particular wavelength excitation there will be fluorescence signal in the reaction zone, the strength of the signal is inversely proportional to the 25-OH-Vit-D content in the sample.
Vitamin D is a fat-soluble steroid derivative, and vitamin D is derived from sunlight for the skin. Skin synthesis of vitamin D is transported to the liver by binding to the vitamin D binding protein, while the absorbed vitamin D is transported to the liver by conjugation with chylomicrons and lipoproteins. Vitamin D deficiency is prevalent in the world, affecting 30% to 50% of people. 25-hydroxyvitamin D (25OHD) determination is the best indicators for measuring vitamin D nutritional status.This kit is the quantitative detection of content of 25-hydroxyvitamin D (25-OH-Vit-D) in human serum samples.