* For research use only, not for use in diagnostic procedures.
The Rapid Aflatoxin M1 Test is a qualitative one-step competitive inhibition immunoassay for the detection of aflatoxin. It detects the presence of aflatoxin M1 at 0.2 ng/mL or higher in milk samples by utilizing highly specific reactions between antibodies and aflatoxin M1 in milk samples.
Aflatoxin is a potent liver toxin known to cause cancer in animals. In swine, aflatoxin can cause reduced weight gain, reduced Abraxislity to resist diseases, hepatitis and death. The Food and Drug Administration (FDA) has established action levels of 20 parts per billion (ppb) for grain and feed products, and 0.5 ppb for milk. Grain, feed, or milk containing aflatoxin at or above these levels cannot be sold for food or feed in interstate sales.
Principle Of The Test
Competitive assays are primarily used for testing small molecules. If Aflatoxin M1 are present in the sample it will therefore bind with the conjugate and will be labelled. As the sample migrates along the membrane and reaches the capture zone an excess of labelled antibody will bind to the immobilised antigen so that no visible line is produced. The bound conjugate will then bind to the antibodies in the control zone producing a visible control line. A single control line on the membrane is a positive result. Two visible lines in the capture and control zones is a negative result. However, if an excess of unlabelled Aflatoxin M1 are not present, a weak line may be produced in the capture zone, indicating an inconclusive result.