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* For research use only, not for use in diagnostic procedures.
Intended Use
Product Introduction
General Description
Alpha Fetoprotein is a fetal-specific glycoprotein with a molecular weight of around 70 kDa. It is expressed in the embryonic liver, by cells of the vitelline sac and by the fetal intestinal tract in the first trimester of pregnancy. After birth the synthesis of alpha Fetoprotein decreases rapidly. Alpha Fetoprotein level in adults is low but detectable. Alpha Fetoprotein has no known function in healthy adults. A high level of alpha Fetoprotein in adult individuals may be associated with Hepatocellular carcinoma (HCC), malignant tumor of the liver. Thus, the concentration of alpha Fetoprotein in serum can be measured as a first step in HCC diagnosis.
Principle Of The Test
The test is based on the principle of sandwich immunoassay. Monoclonal and polyclonal antibodies are employed to identity AFP specifically. This test takes only 10-20 minutes to detected 25 ng/mL AFP in serum.
Assay Procedure
1. When you are ready to begin testing, open the sealed pouch by tearing along the notch. Remove the test kit from the pouch and use it as soon as possible.
2. Draw 0.2 mL (about 3-4 drops) sample using the pipette, and dispense it into the sample well on the cassette.
3. Wait 10-20 minutes and read result. It is important that the background is clear before the result is read. Do not read results after 30 minutes.
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