The CDIATM Amphetamine Colloidal Gold Test Cassette (Urine) is a lateral flow chromatographic immunoassay for the detection of amphetamine and its metabolites in human urine.
Amphetamine antigen is coated on the test region of the nitrocellulose membrane of the strips, and amphetamine antibody is labeled with colloid gold. During a test, the colloid gold labeled antibody coated in the strip move forward along the membrane, and a red line will show up when the antibody gathers with the antigen in the test line; if amphetamine in the sample is over the detection limit, line T is blank; on the other wise, if amphetamine in the sample is less than the detection limit, line T is red.
Amphetamines are central nervous system stimulants that produce alertness, wakefulness, increased energy, reduced hunger, and overall feeling of well-being. They are chemically related to the human body's natural catecholamines: epinephrine and norepinephrine. Large doses and extended usage can result in higher tolerance levels and physiological dependency leading to substance abuse. The effect of Amphetamines generally last 2-4 hours following use, and the drug has a half-life of 4-24 hours in the body. About 30% of Amphetamines are excreted in the urine in unchanged form, with the remainder as hydroxylated and deaminated derivatives. The CDIATM Amphetamine Colloidal Gold Test Cassette (Urine) is a rapid urine-screening test that can be performed without the use of an instrument. The test utilizes a monoclonal antibody to selectively detect elevated levels of amphetamine in urine. The CDIATM Amphetamine Colloidal Gold Test Cassette (Urine) yields a positive result when the amphetamine in urine exceeds 2000/1000/500/300 ng/mL.