Barbiturates are central nervous system depressants. They are used therapeutically as sedatives, hypnotics, and anticonvulsants. Barbiturates are almost always taken orally as capsules or tablets. The effects resemble those of intoxication with alcohol. Chronic use of Barbiturates leads to tolerance and physical dependence. Short acting Barbiturates taken at 400 mg/day for 2-3 months produces a clinically significant degree of physical dependence. Withdrawal symptoms experienced during periods of drug abstinence can be severe enough to cause death. Only a small amount (less than 5%) of most Barbiturates are excreted unaltered in the urine. The detection period for the Barbiturates in the urine is 4-7 days.
The CDIATM Barbiturates (BAR) Test Strip is a rapid urine-screening test that can be performed without the use of an instrument. The test utilizes a monoclonal antibody to selectively detect elevated levels of Barbiturates in urine. The CDIATM Barbiturates (BAR) Test Strip yields a positive result when the Barbiturates in urine exceeds the cut-off level.
CDIATM Barbiturates (BAR) Test Strip is an immunoassay based on the principle of competitive binding. Drugs that may be present in the urine specimen compete against the drug conjugate for binding sites on the antibody.
During testing, a urine specimen migrates upward by capillary action. Barbiturates, if present in the urine specimen below the cut-off level, will not saturate the binding sites of the antibody in the test. The antibody coated particles will then be captured by immobilized Barbiturates-protein conjugate and a visible colored line will show up in the test line region. The colored line will not form in the test line region if the Barbiturates level exceeds the cut-off level, because it will saturate all the binding sites of anti-Barbiturates antibodies.