The CDIATM Barbiturates Colloidal Gold Test Cassette (Urine) is a lateral flow chromatographic immunoassay for the detection of barbiturates and its metabolites in human urine.
Barbiturates antigen is coated on the test region of the nitrocellulose membrane of the strips, and barbiturates antibody is labeled with colloid gold. During a test, the colloid gold labeled antibody coated in the strip move forward along the membrane, and a red line will show up when the antibody gathers with the antigen in the test line; if barbiturates in the sample is over the detection limit, line T is blank; on the other wise, if barbiturates in the sample is less than the detection limit, line T is red.
Barbiturates are central nervous system depressants. They are usually administered orally but are sometimes injected intramuscularly and intravenously. Barbiturates range from short-acting (approximately 15 minutes, such as secobarbital) to long-acting (24 hours or longer, such as Phenobarbital). Short-acting barbiturates are extensively metabolized in the body, while the long-acting ones are secreted primarily unchanged. Barbiturates produce alertness, wakefulness, increased energy, reduced hunger, and an overall feeling of well being. Large doses of Barbiturate could develop tolerance and physiological dependency and lead to its abuse. The CDIATM Barbiturates Colloidal Gold Test Cassette (Urine) is a rapid urine-screening test that can be performed without the use of an instrument. The test utilizes a monoclonal antibody to selectively detect elevated levels of barbiturates in urine. The CDIATM Barbiturates Colloidal Gold Test Cassette (Urine) yields a positive result when the barbiturates in urine exceeds 600/300/200 ng/mL.