The CDIATM Benzodiazepines Colloidal Gold Test Cassette (Urine) is a lateral flow chromatographic immunoassay for the detection of benzodiazepines and its metabolites in human urine.
Benzodiazepines antigen is coated on the test region of the nitrocellulose membrane of the strips, and benzodiazepines antibody is labeled with colloid gold. During a test, the colloid gold labeled antibody coated in the strip move forward along the membrane, and a red line will show up when the antibody gathers with the antigen in the test line; if benzodiazepines in the sample is over the detection limit, line T is blank; on the other wise, if benzodiazepines in the sample is less than the detection limit, line T is red.
Benzodiazepines are a class of drugs that are often therapeutically used as anxiolytics, anti-convulsants and sedative hypnotics. Benzodiazepines manifest their presence by analgesia, drowsiness, confusion, diminished reflexes, lowering of body temperature, respiratory depression, blockade of adrenocortical response, and a decrease in peripheral resistance without an impact on the cardiac index. The major pathways of elimination are the kidneys (urine) and the liver where it is conjugated to glucuronic acid. Large doses of Benzodiazepines could develop tolerances and physiological dependency and lead to its abuse. Only trace amounts (less than 1%) of Benzodiazepines are excreted unaltered in the urine, most of Benzodiazepines in urine is conjugated drug. Oxazepam, a common metabolite of many benzodiazepines, remains detectable in urine for up to one week, which makes Oxazepam a useful marker of Benzodiazepines abuse. The CDIATM Benzodiazepines Colloidal Gold Test Cassette (Urine) is a rapid urine-screening test that can be performed without the use of an instrument. The test utilizes a monoclonal antibody to selectively detect elevated levels of benzodiazepines in urine. The CDIATM Benzodiazepines Colloidal Gold Test Cassette (Urine) yields a positive result when the benzodiazepines in urine exceeds 600/300/200 ng/mL.