Calprotectin is a calcium-containing protein that makes up 5% of the total protein and 60% of the cytosolic protein of neutrophil.It has bacteriostatic and fungistatic properties and is found in feces at levels six times higher than that in plasma. That fecal biomarker is useful to assess the activity of inflammatory bowel disease (IBD). IBD includes Crohn's Disease (CD) and Ulcerative Colitis (UC) and are associated with elevated neutrophils. This fecal calprotectin assay is useful in differentiating organic (IBD) from functional gastrointestinal disease (IBS: Intestinal Bowel Syndrome). It is a simple, non-invasive biomarker that is especially useful in children, who may require general anesthesia for colonoscopy. And this fecal calprotectin detection can predict relapse.
Principle Of The Test
The Calprotectin Card is a qualitative immunoassay for the detection of calprotectin in feces samples. The membrane is pre-coated with monoclonal antibodies against calprotectin on the test lines region.During testing, the sample reacts with thecolouredparticles coated with anti-human calprotectin antibodies which were pre-dried on thegold pad. The mixture moves upward on the membrane by capillary action. In the case of a positive result the specific antibodies present on the membrane will react with the mixture conjugate and generate colored line. A green colored band always appears in the control line and serves as verification that sufficient volume was added, that proper flow was obtained and as an internal control for the reagents.