A rapid test for the qualitative detection of Campylobacter spp. in faeces specimens, which might be useful for the diagnosis of campylobacteriosis.
The test showed 99% of sensitivity.
Campylobacteriosis is an infectious disease caused by bacteria of the genusCampylobacter. Most people who become ill with campylobacteriosis get diarrhoea, cramping, abdominal pain, and fever within two to five days after exposure to the organism. The diarrhoea may be bloody and can be accompanied by nausea and vomiting. The illness typically lasts one week. Some infected persons do not have any symptoms. In persons with compromised immune systems,Campylobacter occasionally spreads to the bloodstream and causes a serious life-threatening infection.
Principle Of The Test
The CAMPYLOBACTER SPECIES Ag CARD is a qualitative immunochromatographic assay for the determination of Campylobacter spp. in faeces samples. The membrane is pre-coated with monoclonal antibodies, on the test band region, againstCampylobacter antigens. During testing, the sample is allowed to react with the coloured conjugate (anti-Campylobacter monoclonal antibodies-red polystyrene microspheres) which was pre-dried on the test strip. The mixture then moves upward on the membrane by capillary action. As the sample flows through the test membrane, the coloured particles migrate. In the case of a positive result the specific antibodies present on the membrane will capture the coloured conjugate. The mixture continues to move across the membrane to the immobilized antibody placed in the control band region, a green coloured band always appears. The presence of thisgreen band serves as 1) verification that sufficient volume is added, 2) that proper flow is obtained and 3) as an internal control for the reagents.