Chagas Ab Rapid Test (Serum) is an immunochromatographic test designed to detect antibodies specific for Trypanosoma cruzi in serum. This protozoan is the causal agent for Trypanosomiasis or Chagas disease.
Serum, plasma, whole blood
Given that there is a post-infection window in which antibody concentration is too low to be detected, a negative result cannot exclude the possibilit
Trypanosoma cruzi is a species of parasitic euglenoid trypanosomes. This species causes the trypanosomiasis diseases in humans and animals in America. Transmission occurs when the reduviid bug deposits feces on the skin surface and
subsequently bites; the human host then scratches the bite area, which facilitates penetration of the infected feces. Human American trypanosomiasis, or Chagas disease, has two forms, a trypomastigote found in human blood and an amastigote found in tissues. The acute form usually goes unnoticed and may present as a localized swelling at the site of entry. The chronic form may develop 10 to 20 years after infection. This form affects internal organs (e.g., the heart, the esophagus, the colon, and the peripheral nervous system). Affected people may die from heart failure.
Principle Of The Test
Chagas Ab Rapid Test (Serum) is an immunochromatographic test capable of detecting antibodies specific for various immunologically-relevant Trypanosoma cruzi antigens. The test is based on the immunological capture of microparticles that are colored and covered with a multi-antigen protein as they pass through a membrane on which the same multi-antigen protein has been immobilized. If the sample contains antibodies against T. cruzi, they react with the RED colloid particles that are conjugated to the multiantigen protein. The colloid particle-antibody complexes then migrate through chromatography to the reaction area. In this area, the multi-antigen protein representing T. cruzi has been deposited on a first line; the protein captures the red particle-antibody complexes, producing a RED band. Whether the sample is negative or positive, the appearance of a BLUE band verifies that chromatography has occurred correctly and under conditions that ensure the antigen-antibody reaction.