The CDIATM Cocaine Colloidal Gold Test Cassette (Urine) is a lateral flow chromatographic immunoassay for the detection of cocaine and its metabolites in human urine.
Cocaine antigen is coated on the test region of the nitrocellulose membrane of the strips, and cocaine antibody is labeled with colloid gold. During a test, the colloid gold labeled antibody coated in the strip move forward along the membrane, and a red line will show up when the antibody gathers with the antigen in the test line; if cocaine in the sample is over the detection limit, line T is blank; on the other wise, if cocaine in the sample is less than the detection limit, line T is red.
Cocaine is an alkaloid present in Coca leaves (Erythyroxine coca). Its pharmacological properties, such as stimulating and euphoric effects, have been known for centuries. Cocaine produces alertness, wakefulness, increased energy, reduced hunger, and an overall feeling of well being. In large dose, Cocaine causes fever, unresponsiveness, difficulty in breathing and unconsciousness. Cocaine is often self-administered by nasal inhalation, intravenous injection and free-base smoking. Cocaine is excreted in the urine primarily as Benzoylecgonine, which can generally be detected for 24–48 hours after cocaine exposure. The CDIATM Cocaine Colloidal Gold Test Cassette (Urine) is a rapid urine-screening test that can be performed without the use of an instrument. The test utilizes a monoclonal antibody to selectively detect elevated levels of cocaine in urine. The CDIATM Cocaine Colloidal Gold Test Cassette (Urine) yields a positive result when the cocaine in urine exceeds 600/300/150 ng/mL.