CDIA^TM Cotinine Colloidal Gold Test Cassette, Urine (DTS665)

The CDIA^TM Cotinine Colloidal Gold Test Cassette (Urine) is a lateral flow chromatographic immunoassay for the detection of cotinine and its metabolites in human urine.

Cotinine antigen is coated on the test region of the nitrocellulose membrane of the strips, and cotinine antibody is labeled with colloid gold. During a test, the colloid gold labeled antibody coated in the strip move forward along the membrane, and a red line will show up when the antibody gathers with the antigen in the test line; if cotinine in the sample is over the detection limit, line T is blank; on the other wise, if cotinine in the sample is less than the detection limit, line T is red.

Cotinine is the first-stage metabolite of nicotine, a toxic alkaloid that produces stimulation of the autonomic ganglia and central nervous system when in humans. Nicotine is a drug to which virtually every member of a tobacco-smoking society is exposed whether through direct contact or second-hand inhalation. In addition to tobacco, nicotine is also commercially available as the active ingredient in smoking replacement therapies such as nicotine gum, transdermal patches and nasal sprays. In a 24-hour urine, approximately 5% of a nicotine dose is excreted as unchanged drug with 10% as Cotinine and 35% as hydroxycotinine; the concentrations of other metabolites are believed to account for less than 5%. The CDIA^TM Cotinine Colloidal Gold Test Cassette (Urine) is a rapid urine-screening test that can be performed without the use of an instrument. The test utilizes a monoclonal antibody to selectively detect elevated levels of cotinine in urine. The CDIA^TM Cotinine Colloidal Gold Test Cassette (Urine) yields a positive result when the cotinine in urine exceeds 200 ng/mL.