The Crypto-Giardia Rapid Test is a one step coloured chromatographic immunoassay for the qualitative detection of Cryptosporidium and Giardia in stool samples.
Giardia and Cryptosporidium are parasites that can be found in water. Giardia causes an intestinal illness called giardiasis. Cryptosporidium is responsible for a similar illness called cryptosporidiosis. These infections have become the most common causes of waterborne diseases (found in both drinking and recreational water) in humans. Giardiasis is a diarrheal illness caused by a very small parasite, Giardia intestinalis (also known as Giardia lamblia and Giardia duodenalis). Once an animal or person is infected with Giardia, the parasite lives in the intestine and is passed in the stool. The parasite is protected by an outer shell and can survive outside the body and in the environment for a long time. The most common symptoms of giardiasis include: diarrhea, loose or watery stool, stomach cramps and upset stomach. Cryptosporidium parvum is the major cause of persistent diarrhoea in developing countries. This parasite is recognised as a highly infectious enteric pathogen and infective stage is transmitted by the fecal- oral route. Symptoms of cryptosporidiosis include watery diarrhoea, stomach cramps, weight loss, nausea and sometimes fever.
Principle Of The Test
The Crypto-Giardia Rapid Test is a qualitative immunochromatographic assay for the determination of Cryptosporidium and Giardia antigens in stool samples. The membrane is pre -coated with antibodies, on the test band region, against these antigens. During testing, the sample is allowed to react with the coloured conjugate (antiCryptosporidium antibodies-red microspheres and anti-Giardia antibodies-blue microspheres) which was pre -dried on the test. The mixture then moves upward on the membrane by capillary action. As the sample flows through the test membrane, the coloured particles migrate. In the case of a positive result the specific antibodies present on the membrane will capture the coloured conjugate. Different coloured lines will be visible, depending upon the virus content of the sample. These lines are used to interpret the result. The mixture continues to move across the membrane to the immobilized antibody placed in the control band region, a GREEN coloured band always appears. The presence of this GREEN band serves as 1) verification that sufficient volume is added, 2) that proper flow is obtained and 3) as an internal control for the reagents.