Cryptosporidium Ag Rapid Test (Stool) chromatographic immunoassay is a procedure for measurement of Cryptosporidium antigens in stool samples.
Cryptosporidium is a genus of protozoans that can cause gastrointestinal illness with diarrhea in humans. Cryptosporidium is the organism most commonly isolated in HIV-positive patients presenting with diarrhea. Treatment is symptomatic, with fluid rehydration, electrolyte correction and management of any pain.
Principle of The Test
Cryptosporidium Ag Rapid Test (Stool) use monoclonal Cryptosporidium parvum-specific antibodies that detect all forms of the parasite's life cycle. The test is based on the use of red microspheres linked covalently to an anti-Cryptosporidium parvum monoclonal antibody, plus blue microspheres as test control. The parasites present in stool samples react with the latex particles which are coated with monoclonal antibodies specific against the antigen. This latex particles/antibodies/parasite complex migrates through a chromatographic process towards the reaction zone. In this zone, there are anti-Cryptosporidium antibodies that react with the latex particles/antibodies/parasite complex. This reaction leads to the appearance of a red line. These lines are used to interpret the result, at five minutes' roomtemperature incubation.