Cucumber green mottle mosaic virus (CGMMV) has a narrow host range which is primarily limited to cucurbit species, the damage it causes to the host plant and fruit can be extensive, resulting in substantial yield losses. Therefore, it is an important threat to fresh market, export and cucurbit seed industries. The virus is easily sap and seed transmissible, and it survives for long periods in infected crop debris. CGMMV symptoms (mottling and mosaic on leaves, fruit mottling and distortion) can be confused with those caused by many other cucurbit viruses, making diagnosis based exclusively on visual symptoms unreliable.
Principle Of The Test
The development of rapid test strips is based on Lateral Flow Device (LFD) technology using specific antibodies. A LFD format consists of antibody-coated latex beads latex (or colloidal gold) which will bind the specific pathogen antigen absorbed from the plant extract. The agglutination is accumulated at a specific location by the presence of a line of target specific antibody, which immobilises the agglutinated latex whilst allowing the background reagents to be washed away by continued flow along the membrane. The membrane contains a strip of target-specific antibody, test strip and a strip of another antibody that binds to the specific antibody, control strip. Latex beads containing bound antigen are trapped on the test strip leaving a visible line. Surplus latex beads that do not contain antigen are trapped on the control strip showing that the assay has worked. This provides a non-subjective and clear read out of a positive result against a low background.