The CDIATM EDDP Colloidal Gold Test Cassette (Urine) is a lateral flow chromatographic immunoassay for the detection of EDDP and its metabolites in human urine.
EDDP antigen is coated on the test region of the nitrocellulose membrane of the strips, and EDDP antibody is labeled with colloid gold. During a test, the colloid gold labeled antibody coated in the strip move forward along the membrane, and a red line will show up when the antibody gathers with the antigen in the test line; if EDDP in the sample is over the detection limit, line T is blank; on the other wise, if EDDP in the sample is less than the detection limit, line T is red.
Methadone, a Schedule II controlled substance, is often used in the treatment of opiate addiction and pain management; it also has a high potential for abuse. Methadone is metabolized primarily into two pharmacologically inactive metabolites, EDDP and EMDP. EDDP represents a better urine marker for monitoring methadone maintenance than testing for un-metabolized methadone alone. The CDIATM EDDP Colloidal Gold Test Cassette (Urine) is a rapid urine-screening test that can be performed without the use of an instrument. The test utilizes a monoclonal antibody to selectively detect elevated levels of EDDP in urine. The CDIATM EDDP Colloidal Gold Test Cassette (Urine) yields a positive result when the EDDP in urine exceeds 300/100 ng/mL.
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