CDIA™ Enterovirus Antigen Test Kit - Creative Diagnostics

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CDIATM Enterovirus Antigen Test Kit (DTS608)

* For research use only, not for use in diagnostic procedures.

Intended Use

The Enterovirus Device is a rapid chromatographic immunoassay for the qualitative detection of Enterovirus antigens (VP1 peptide) in faecal samples to aid in the diagnosis of Enterovirus infection.
  • Stool
  • Kit
  • It was studied 35 stool samples using Enterovirus Device and all of them were confirmed by IDEIA Enterovirus assay (Dako)and IMAGEN Enterovirus (Oxoid

Product Introduction

General Description
Enteroviruses consist of Poliovirus, Coxsakievirus, Echovirus, and numbered Enterovirus. Enteroviruses are single-stranded RNA viruses. Enteroviruses can cause a wide spectrum of diseases in humans. All enteroviruses are transmitted by the fecaloral route, but clinical outcomes may go beyond gastroenteritis, as some viruses travel from the intestinal tract to other organs.Poliovirus usually infects their host by attacking the central nervous system and cause paralysis in victims (poliomyelitis). Coxsackievirus has been associated with not only respiratory system infections and gastroenteritis but also insulin-dependent diabetes and heart diseases, such as myocarditis and pericarditis. Echovirusis generally less infectious than other enteroviruses and are usually associated with the common cold and respiratory diseases. The numbered enteroviruses (Enterovirus types 68 to 71) have not been studied extensively but have been isolated from patients with bronchiolitis, conjunctivitis, meningitis, and paralysis resembling poliomyelitis. Enterovirus Device provides a rapid detection of Enteroviruses directly from the faecal samples.
Principle Of The Test
The Enterovirus Device is a qualitative immunoassay for the detection of VP1 peptide of Enterovirus in faecal samples. The membrane is precoated with mouse monoclonal antibodies against Enterovirus antigen on the test line region. During testing, the sample is allowed to react with the particle conjugate coated with anti-VP1 peptide virus antibodies which was pre-dried on the test strip. The mixture then moves upward on the membrane by capillary action. In the case of a positive result the specific antibodies present on the membrane will react with the mixture conjugate and generate a coloured line. The mixture continues to move across the membrane to the immobilized antibody placed in the controlband region, a GREEN coloured band always appears. The presence of this GREEN band serves as verification that sufficient volume is added,that proper flow is obtained and as an internal control for the reagents.

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