The CDIATM Fatty Acid-binding Protein Immunofluorescence Test Cassette is designed for quantitative determination of fatty acid-binding protein in human serum, whole blood or plasma.
The test uses an anti-human FABP monoclonal antibody conjugated with fluorescence latex and another anti-human FABP monoclonal antibody coated on the test line. After the sample has been applied to the test strip, the fluorescence latex-labelled anti-human FABP monoclonal antibody binds with the FABP in sample and forms a marked antigen-antibody complex. This complex moves to the test card detection zone by capillary action. Then marked antigen-antibody complex is captured on the test line by the anti-human FABP monoclonal antibody. The fluorescence intensity of the test line increases in proportion to the amount of FABP in sample. Then insert test card into the Fluorescent Immunoassay Analyzer FIA7100, the concentration of FABP in sample will be determined and displayed on the screen.
The fatty-acid-binding proteins (FABPs) are a family of transport proteins for fatty acids and other lipophilic substances such as eicosanoids and retinoids. These proteins are thought to facilitate the transfer of fatty acids between extra- and intracellular membranes. Some family members are also believed to transport lipophilic molecules from outer cell membrane to certain intracellular receptors such as PPAR. This kit is intended to use in quantitative detection of fatty-acid-binding proteins content in human serum, plasma or whole blood.