CDIA^TM Hemoglobin/Haptoglobin Test Kit (DTS171)

General Description

Hemoglobin (also spelled haemoglobin and abbreviated Hb or Hgb) is the iron-containing oxygen-transport metalloprotein in the red blood cells of all vertebrates(with the exception of the fish family Channichthyidae) as well as the tissues of some invertebrates. Hemoglobin in the blood carries oxygen from the respiratory organs (lungs or gills) to the rest of the body (i.e. the tissues) where it releases the oxygen to burn nutrients to provide energy to power the functions of the organism, and collects the resultant carbon dioxide to bring it back to the respiratory organs to be dispensed from the organism. Haptoglobin (abbreviated as Hp) is a protein that in humans is encoded by the HP gene. In blood plasma, haptoglobin binds free hemoglobin (Hb) released from erythrocytes with high affinity and thereby inhibits its oxidative activity. The haptoglobin-hemoglobin complex will then be removed by the reticuloendothelial system (mostly the spleen). In clinical settings, the haptoglobulin assay is used to screen for and monitor intravascular hemolytic anemia. In intravascular hemolysis free hemoglobin will be released into circulation and hence haptoglobin will bind the Hb. This causes a decline in Hp levels. Conversely, in extravascular hemolysis the reticuloendothelial system, especially splenic monocytes, phagocytose the erythrocytes and hemoglobin is not released into circulation; serum haptoglobin levels are therefore normal.

Assay Procedure

1. Bring the extracted sample to room temperature if it is refrigerated. Then mix the extracted sample well by shaking the sample collection device a few times.
2. Remove the test cassette from the foil pouch, and place it on a flat, dry surface.
3. If the stool sample is refrigerated, then bring the sample collection device to room temperature. Then shake the device several times.
4. Hold the sample collection device so that the device tip facing up, then break off the tip of the collection device. Squeeze 3 drops of the extracted sample into each sample well.
5. As the test kit begins to work, you will see a purple coloured front move across the Result Window in the centre of the test cassette.
6. Interpret test results at 10 minutes. Do not read after more than 15 minutes.
CAUTION: The above interpretation time is based on reading the test results at room temperature of 15 to 30°C. If your room temperature is significantly lower than 15°C, then the interpretation time should be properly increased