CDIA™ Hepatitis A Virus Antigen Test Kit - Creative Diagnostics

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CDIATM Hepatitis A Virus Antigen Test Kit (DTS597)

* For research use only, not for use in diagnostic procedures.

Intended Use

The HAV Ag CARD is a rapid chromatographic immunoassay for the qualitative detection of Hepatit is A antigens in faecal samples to aid in the diagnosis of HAV infection.
  • Stool
  • Kit
  • The results showed 99% of sensitivity.

Product Introduction

General Description
The Hepatitis A virus is a single-stranded RNA virus that belongs to the Picornaviridae family of viruses. The majority of acute HAV infections are subclinical. When symptoms do appear, they tend to be mild and non-specific in nature. Most commonly they include fever, general malaise, fatigue, abdominal discomfort and change in bowel habits. When severe, dark urine, pale stool and jaundice may appear. The severity of acute HAV infections is proportional to the age of the patient, with younger patients tending to have milder disease than the elderly. HAV is spread through faecal contamination of food or drinking water. Although the virus is present in blood, the limited amount of circulating virus and short duration of viremia render parenteral transmission of this virus extremely uncommon. Faeces of infected individuals tend to contain the virus for a 2-week period prior to the onset of illness and for at least 2 weeks and perhaps as long as 3 months thereafter. Acute hepatitis A is one of the well known vaccine preventable diseases and active Hepatitis A virus (HAV) vaccination is recommended in high risk populations. HAV Ag CARD provides a rapid detection of Hepatitis A directly from the faecal samples.
Principle Of The Test
The HAV Ag CARD is a qualitative lateral flow immunoassay for the detection of Hepatitis A virus antigen in faecal samples. The membrane is pre-coated with mouse monoclonal antibodies against hepatitis A viral antigens on the test line region. During testing, the sample is allowed to react with the particle conjugate coated with anti-Hepatitis A virus antibodies which was predried on the test strip. The mixture then moves upward on the membrane by capillary action. As the sample flows through the test membrane, the coloured particles migrate. In the case of a positive result the specific antibodies present on the membrane will react with the mixture conjugate and generate a coloured line. The mixture continues to move across the membrane to the immobilized antibody placed in the control band region, a GREEN coloured band always appears. The presence of this GREEN band serves as verification that sufficient volume is added, that proper flow is obtained and as an internal control for the reagents.

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