Although a wide variety of viral agents are capable of causing lower respiratory tract infections in children and adults, influenza A & B; respiratory syncytial virus (RSV); parainfluenza viruses 1, 2, and 3; and adenovirus are the most common. Symptoms of respiratory illness caused by adenovirus infection range from the common cold syndrome to pneumonia, croup, and bronchitis.
The Adenovirus Resp. Rapid test Card is a qualitative lateral flow immunoassay for the detection of Adenovirus antigen in human nasopharyngeal samples. The membrane is pre-coated with monoclonal antibodies against Adenovirus antigens on the test line region. During testing, the sample reacts with the particle coated with anti-Adenovirus antibodies which was pre-dried on the test strip. The mixture moves upward on the membrane by capillary action. In the case of a positive result the specific antibodies present on the membrane will react with the mixture conjugate and generate a coloured line. A green coloured line always appears in the control line and serves as verification that sufficient volume was added, that proper flow was obtained and as an internal control for the reagents.
Figure 1. Assay procedure (collected nasopharyngeal swab)
Figure 2. Assay procedure (collected nasopharyngeal wash or aspirate samples)
Figure 3. Interpretation of results
Reagents And Materials Provided
1. Rapid Adeno Resp. Card tests (Plastic pipettes included)
2. Instructions for use
3. Diluent (sample diluent)
5. Testing tubes or vials
6. Adenovirus Respiratory Control swabs