Immunochromatographic card test for the qualitative detection of Adenovirus and/or Rotavirus in human faeces.
The detection of Rotavirus showed 99% of concordance in sensitivity. The detection of Adenovirus showed 99% of concordance in sensitivity.
Rotavirus and Adenovirus are major causes of infectious gastroenteritis in infants and young children, also observed in adults. They are transmitted by fecal-oral contact. The main symptoms of viral gastroenteritis are watery diarrhoea and vomiting. The affected person may also have headache, fever, and abdominal cramps ("stomach ache"). In general, the symptoms begin 1 to 2 days following infection with a virus that causes gastroenteritis and may last for 1 to 10 days, depending on which virus causes the illness (Rotavirus 3 days and Adenovirus 5-8 days).
Principle Of The Test
The Rotavirus-Adenovirus is a qualitative immunochromatographic assay for the determination of Rotavirus and Adenovirus in feces samples. The membrane is pre-coated with mouse monoclonal antibodies, onthe test band region, against viral antigens. During testing, the sample is allowed to react with the coloured conjugate (anti-rotavirus mouse monoclonal antibodies-red microspheres and anti-adenovirus mouse monoclonalantibodies-blue microspheres) which was pre-dried on the test. The mixture then moves upward on the membrane by capillary action. As the sample flows through the test membrane,the coloured particles migrate. In the case of a positive result the specific antibodies present on the membrane will capture the coloured particles. Different coloured lines will be visible,depending upon the virus content of the sample. These lines are used to interpret the result. The mixture continues to move across the membrane to the immobilized antibody placed inthe control band region, a GREEN coloured band always appears. The presence of this GREEN band serves as 1) verification that sufficient volume is added, 2) that proper flow is obtained and 3) as an internal control for the reagents.