The Astrovirus Deviceis a rapid chromatographic immunoassay for the qualitative detection of Astrovirus antigens in human faeces specimens to aid in the diagnosis of Astrovirus infection.
The evaluation was conducted comparing the results obtained using theAstrovirus Device to a commercial availableAstrovirus ELISA assay.The detection
Viral gastroenteritis is an infection caused by a variety of viruses thatresults in vomiting or diarrhea. Many different viruses can cause gastroenteritis, including rotaviruses, noroviruses, adenoviruses, sapoviruses, and astroviruses. The main symptoms of viral gastroenteritis are watery diarrhea and vomiting. The affected person may also have headache, fever, and abdominal cramps ("stomach ache"). In general, the symptoms begin 1 to 2 days following infection with a virus that causes gastroenteritis and may last for 1 to 10 days, depending on which virus causes the illness. Some research studies have shown that the duration of the symptoms are approximately three to four days. Astrovirus infection is not usually a severe situation and only in some rare cases leads to dehydration. Adenoviruses and astroviruses cause diarrhea mostly in young children, but older children and adults can also be affected.
Principle Of The Test
TheAstrovirus Device is a qualitative immunoassay for the detection of Astrovirus antigen in human faeces samples. The membrane is precoated with monoclonal antibodies against Astrovirus antigens on the test line region. During testing, the sample reacts with the particle coated with anti-Astrovirus antibodies which was pre-dried on the test strip. The mixture moves upward on the membrane by capillary action. In the case of a positive result the specific antibodies present on the membrane will react with the mixture conjugate and generate a coloured line. A green coloured band always appears in the control line and serves as verification that sufficient volume was added, that proper flow was obtained and as an internal control for the reagents.