Human chronic gonadotropin (hCG) is a glycoprotein hormone secreted by the developing placenta shortly after fertilization. The hCG appears soon after conception and only during early stages of pregnancy. The CD hCG Rapid Test is a qualitative, two-site sandwich immunoassay for the determination of hCG antigen. The assay employs a combination of hCG monoclonal antibodies to selectively detect elevated levels of hCG in human urine specimens. The immunological specificity of the test kit virtually eliminates cross reactivity with the structurally related glycoprotein hormones hFSH, hLH and hTSH at physiological levels.
Principle Of The Test
The CD hCG Rapid Test is a chromatographic immunoassay (CIA) for the rapid qualitative determination of hCG in human urine specimens. The membrane is precoated with hCG specific antibodies on the test region. During the test, the specimen is allowed to react with the hCG monoclonal antibody-colloid gold conjugate pre-dried on the test strip. The mixture then moves upward on the membrane chromatographically by capillary action. For a positive specimen, the conjugate binds to the hCG forming an antibody-antigen complex. This complex binds to hCG antibody as a capture regents on the test region and produces a colored band when hCG concentration is equal to or greater than 20 mIU/ml. Absence of this colored band in the test region suggests a negative result. To serve as a procedural control, a colored band in the control region will always appeared regardless the presence of hCG.