The Crypto Device test is a rapid chromatographic immunoassay for the qualitative detection of Cryptosporidium parvum antigens in human faeces specimens to aid in the diagnosis of cryptosporidiosis.
The result showed using Cryp to Device: 99% of sensitivity.
Cryptosporidiosis is a diarrhoeal disease caused by microscopic parasites of the genus Cryptosporidium. Once an animal or person is infected, the parasite lives in the intestine and passes in the stool. The parasite is protected by an outer shell that allows it to survive outside the body for long periods of time and makes it very resistant to chlorine-based disinfectants. Both the disease and the parasite are commonly known as "Crypto."
Principle Of The Test
The Crypto Device is a qualitative lateral flow immunoassay for the detection of Cryptosporidium antigen in human faeces samples. The membrane is pre-coated with antibodies against Cryptosporidiumantigens on the test line region. During testing, the sample reacts with the particle coated with anti-Cryptosporidium antibodies which was pre-dried on the test strip. The mixture moves upward on the membrane by capillary action. In the case of a positive result the specific antibodies present on the membrane will react with the mixture conjugates and generate one or two coloured lines. A green coloured band always appears in the control line (third line) and serves as verification that sufficient volume was added, that proper flow was obtained and as an internal control for the reagents.