Entamoeba Ag Rapid Test chromatographic immunoassay is a procedure for measurement of Entamoeba histolytica antigens in human stool samples.
Entamoeba is a genus of Amoebozoa found as internal parasites or commensals of animals. In 1875, Fedor Lösch described the first proven case of amoebic dysentery in St Petersburg, Russia. He referred to the amoeba he observed microscopically as 'Amoeba coli'; however it is not clear whether he was using this as a descriptive term or intended it as a formal taxonomic name. The genus Entamoeba was defined by Casagrandi and Barbagallo for the species Entamoeba coli, which is known to be a commensal organism. Lösch's organism was renamed Entamoeba histolytica by Fritz Schaudinn in 1903; he later died, in 1906, from a self-inflicted infection when studying this amoeba. For a time during the first half of the 20th century the entire genus Entamoeba was transferred to Endamoeba, a genus of amoebas infecting invertebrates about which little is known. This move was reversed by the International Commission on Zoological Nomenclature in the late 1950s, and Entamoeba has stayed 'stable' ever since.
Principle Of The Test
Entamoeba Ag Rapid Test use monoclonal antibodies specific for Entamoeba histolytica that detect all forms of the parasite during its life cycle. The test is based on the use of red microspheres covalently linked to an anti-Entamoeba histolytica monoclonal antibody, plus blue microspheres as test control. The parasites present in stool samples react with the latex particles which are coated with specific monoclonal antibodies against the antigen. This latex particles/antibodies/parasite complex migrates through a chromatographic process towards the reaction area. In this area, anti-Entamoeba histolytica antibodies that react with the latex particles/antibodies/parasite complex are present. This reaction leads to the appearance of a red line. These lines are used to interpret the result after a five-minute incubation at room temperature.