The H. Pylori Rapid Test is a chromatographic immunoassay (CIA) for the rapid determination of antibodies to H. pylori in human serum and/or whole blood specimens.
Serum and Whole blood
Helicobacter pylori (H. pylori), previously named Campylobacter pyloridis, is a Gram-negative, microaerophilic bacterium found in the stomach. It was identified in 1982 by Barry Marshall and Robin Warren, who found that it was present in patients with chronic gastritis and gastric ulcers, conditions that were not previously believed to have a microbial cause. It is also linked to the development of duodenal ulcers and stomach cancer. However, over 80 percent of individuals infected with the bacterium are asymptomatic and it has been postulated that it may play an important role in the natural stomach ecology.
Principle Of The Test
The CD H. pylori Antibody Rapid Test is a lateral flow, immunochromatographic screening test. H. pylori specific antigens are precoated onto membrane as a capture reagent on the test band region. During the assay the the whole blood or serum specimen is first allowed to react with H. pylori specific antigen-gold conjugate complexes. The mixture then moves laterally on the membrane to the test region which is coated with immobilized antibodies to H. pylori. If H. pylori antibodies are present in the specimen, a color band is formed on the test (T) region. Absence of the colored band in the test region indicates a negative result. To serve as a procedural control, a colored band in the control (C) region will always appear regardless the presence of H. pylori antibodies in the whole blood and/or serum specimen.