CDIA™ Human Influenza A (Swine Flu) Rapid Test - Creative Diagnostics

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Infectious Disease Tests | Influenza A virus |

CDIATM Human Influenza A (Swine Flu) Rapid test (DTSXYZ9)

Intended Use

The Influenza A (Swine Flu) test is a rapid chromatographic immunoassay for the qualitative detection of type A Influenza antigens in human nasopharyngeal specimens (swab, nasopharyngeal wash and aspirate) and in pig specimens (nasal, lung tissue or feces), to aid in the diagnosis of swine flu infection in humans and in pigs, respectively.
  • Nasal swabs, Washes, Aspirates, Pig specimen
  • Kit
  • 99%
  • 99%

Product Introduction

General Description

Influenza A virus, of the family Orthomyxoviridae, carries an RNA genome. This genome encodes one or two non-structural proteins and nine structural proteins, which, together with a host cell–derived lipid envelope, comprise the influenza virus particle. Influenza virus causes widespread morbidity and mortality among human populations worldwide. Swine influenza is known to be caused by influenza A subtypes H1N1, H1N2, H3N1, H3N2, and H2N3. In pigs, three influenza A virus subtypes (H1N1, H3N2, and H1N2) are the most common strains worldwide. The symptoms of swine flu are cough, fever, sore throat, fatigue, decreased appetite, less commonly vomiting and diarrhoea.

Principle

The Influenza A (Swine Flu) Rapid test is a qualitative lateral flow immunoassay for the detection of type A Influenza antigen in human and pig nasopharyngeal samples and pig stool and lung-tissue samples. The membrane is pre-coated with monoclonal antibodies against Influenza type A antigens on the test line region. During testing, the sample reacts with the particle coated with anti-Influenza antibodies which was pre-dried on the test strip. The mixture moves upward on the membrane by capillary action. In the case of a positive result the specific antibodies present on the membrane will react with the mixture conjugate and generate one coloured lines. A green coloured band always appears in the control line and serves as verification that sufficient volume was added, that proper flow was obtained and as an internal control for the reagents.

Figure 1. Assay procedure (collected nasopharyngeal wash or aspirate samples)




           Figure 2. Assay procedure (collected nasopharyngeal swab)



Figure 3. Assay procedure (tissue (supernatant)/stool collection sample)



                                  Figure 4. Interpretation of results


Reagents And Materials Provided

1. Card tests contained plastic pipettes 

2. Instructions for use 

3. Testing tubes or vials 

4. Diluent B (REAG) 

5. Tissue/stool collection vial with buffer 

6. Sterile swabs 

7. Certificate of package control

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