The Lactoferrin Blister Rapid Test is a rapid chromatographic immunoassay for the qualitative detection of human lactoferrin in faecal samples that may reflect intestinal inflammation in inflammatory bowel disease (IBD).
A sample containing lactoferrin at concentration equal to or higher than 10ug hLf/g feces produces positive results when usingthe Lactoferrin Blister
Principle Of The Test
The Lactoferrin Blister Rapid Test is a qualitative immunochromatographic assay for the determination of human lactoferrin in faecal samples. The membrane is pre-coated with antibodies on the test band (result region), against human lactoferrin. During testing, the sample is allowed to react with the coloured conjugate (anti-human lactoferrin antibodies-red microspheres) pre-dried on the test. The mixture then moves upward on the membrane by capillary action. As the sample flows through the test membrane, the coloured particles migrate. In the case of a positive result the specific antibodies present on the membrane will capture the coloured conjugate. A coloured band will be visible, depend on the lactoferrin content of the sample. This band is used to interpret the result. The mixture continues to move across the membrane to the immobilized antibody placed in the control band region; this red coloured band always appears. The presence of this red band serves as verification that sufficient volume is added, that proper flow obtained and as an internal control for the reagents.