Clinical syndromes in humans caused by infection with Salmonella enterica are divided into typhoid fever, caused by S. enterica serovars typhi and paratyphi, and a range of clinical syndromes, including diarrhoeal disease, caused by the non-typhoid Salmonellae (NTS) of which there are around 2,500 serovars. Typhoid fever is a human-restricted and highly adapted invasive systemic disease of adults and children that shows little association with immunosuppression. In contrast, NTS have a broad vertebrate host range and epidemiology that often involves food animals. Severe, invasive disease due to NTS is usually associated with the immunocompromised state common in HIV-infected adults. The Salmonella Typhi Rapid test kit provides a rapid detection of Salmonella typhi directly from the fecal samples.
The Salmonella Typhi Rapid test is a qualitative lateral flow immunoassay for the detection of Salmonella typhi antigens in human feces samples. The membrane is pre-coated with monoclonal antibodies against Salmonella typhi antigens on the test line region. During testing, the sample reacts with the particle coated with anti-S. typhi antibodies which was pre-dried on the test strip. The mixture moves upward on the membrane by capillary action. In the case of a positive result, a red color line will be visible in the result line region. Whether there is presence of S. typhi or not, the mixture continues to move across the membrane to the immobilized antibody placed in the control band region and a green coloured band always appears (control line). The presence of this line serves as: verification that sufficient volume was added, that proper flow was obtained and as an internal control for the reagents.
Figure 1. Assay procedure (collected stool samples)
Figure 2. Interpretation of results
Reagents And Materials Provided
1. Rapid Salmonella typhi Card tests
2. Instructions for use
3. Specimen collection vial with buffer