Clinical syndromes in humans caused by infection with Shigella are divided into S. dysenteriae, S. flexneri, S. boydii and S. sonnei, and a range of clinical syndromes, including diarrhoeal disease, fever and stomach cramps. Shigellosis are associated with poor sanitation, contaminated food and water, and crowded living conditions and usually resolves in 5 to 7 days. The predominant serogroups of Shigella are particularly common and it causes recurrent problems in settings where hygiene is poor and can sometimes sweep through entire communities. The Shigella Rapid test kit provides a rapid detection of Shigella directly from the fecal samples.
The Shigella Rapid test is a qualitative lateral flow immunoassay for the detection of Shigella antigens in fecal samples. The membrane is pre-coated with monoclonal antibodies against Shigella antigens on the test line region. During testing, the sample reacts with the particle coated with anti-Shigella antibodies which was pre-dried on the strip. The mixture moves upward on the membrane by capillary action. In the case of a positive result the specific antibodies present on the membrane will react with the mixture conjugate and generate a coloured line. A green coloured line always appears in the control line and serves as verification that sufficient volume was added, that proper flow was obtained and as an internal control for the reagents.
Figure 1. Assay procedure (collected stool samples)
Figure 2. Interpretation of results
Reagents And Materials Provided
2. Specimen collection vials with buffer
3. Instructions for use