* For research use only, not for use in diagnostic procedures.
Immunochromatographic test for the qualitative detection of total antibodies against Echinococcus granulosus in serum/plasma
Echinococcus granulosus is the causal agent of human hydatidosis. The adult phase parasites the small intestine of cannids (definitive hosts), while the larva affects sheep and cattle (intermediate hosts) and, in a secondary or accidental way, other animals included humans. When the larval phase infects internediate host, hydatidic cysts develop in their internal organs, most often located in the liver (mainly in the right lobule), by the lung and other organs, such as the brain and bones. The intensity of the immune response depends on the location and integrity of the cyst. Cysts in liver and bone are more reactive than those in lung, brain or spleen. Serologic assays, together with imaging techniques, are most frequently used for the diagnosis of hydatidosis. Cross-reactions may appear in patients infected by other helminths (mainly with cysticercosis) and in oncological processes. A HPLC-purified E. granulosus 5/B enriched antigen is used in CD HYDATIDOSIS to improve the specificity of the assay while keeping a high sensitivity.
Principle Of The Test
When the sample is added into the well of the cassette, the colloidal gold is solubilized and the first immunological reaction between the specific antibodies of the serum/plasma and the protein coupled to the gold particles takes place. These complexes move along the membrane to the reaction line (test line), and a colored band will appear if the analyte to be detected is present in the sample. Each strip contains a control line for the validation of the assay. This line has to appear always even if the sample is negative.