Immunochromatographic test for the qualitative detection of Legionella genus, Legionella pneumophila serogroup 1 and Legionella pneumophila serogroups 1-15 in samples from bacterial cultures obtained from environmental samples.
It is recommended to read carefully the “Precautions” section. CD LEGIONELLA CULTURE enables to identify the main Legionella groups of environmental importance in a single assay. The test is accomplished in minutes with the help of materials and equipment commonly found in any analysis laboratory and can be read visually. The reactivity against other species and serogroups not specifically tested is supported by the use of Mabs against common antigens.
LEGIONELLA CULTURE is an immunoassay for the qualitative detection of Legionella genus, specie and serogroup. This test is for professional use and helps to the determination of the type of Legionella found in a bacterial culture. These samples are of environmental origin. L. pneumophila serogroup 1 alone causes 80% of the cases of legionellosis and is most frequently isolated from water samples collected from building facilities. It causes atypical pneumonia, frequently with systemic manisfetations. It is responsible for 10% cases of pneumonia acquired in both the community and hospitals. During the infection, an immune response is developed against the L. pneumophila group related antigen. Nevertheless, the presence of other species of Legionella in the water is also considered as a potential risk. Species such as Legionella micdadei, Legionella dumoffi, Legionella bozemanii, Legionella feeli and Legionella longbeachae appear often in facilities of water supply and have caused some declared outbreaks. As a consequence, a test able to classify the Legionella isolates from culture will help to elucidate its potential pathogenicity. In order to classify each Legionella isolate, monoclonal antibodies (Mab) against different components of the bacterial wall have been used. Mabs against PAL (peptidoglycan–associated-lipoprotein) common in all Legionella species have been used for the genus detection; Mabs against a common epitope of MOMP (major outer membrane protein) present in all the L. pneumophila serogroups have been used for the species (serogroups 1-15) identification, and Mabs against LPS (lipopolysaccharide) have been used for L. pneumophila serogroup 1 characterization.