The CDIATM Methadone Colloidal Gold Test Cassette (Urine) is a lateral flow chromatographic immunoassay for the detection of methadone and its metabolites in human urine.
Methadone antigen is coated on the test region of the nitrocellulose membrane of the strips, and methadone antibody is labeled with colloid gold. During a test, the colloid gold labeled antibody coated in the strip move forward along the membrane, and a red line will show up when the antibody gathers with the antigen in the test line; if methadone in the sample is over the detection limit, line T is blank; on the other wise, if methadone in the sample is less than the detection limit, line T is red.
Detoxification using methadone can either be done relatively rapidly in less than a month or gradually over as long as six months. While a single dose has a rapid effect, maximum effect can take five days of use. The effects last about six hours after a single dose and a day and a half after long-term use in people with normal liver function. Methadone is usually taken by mouth and rarely by injection into a muscle or vein. Side effects are similar to those of other opioids. Commonly these include dizziness, sleepiness, vomiting, and sweating. Serious risks include opioid abuse and a decreased effort to breathe. Abnormal heart rhythms may also occur including prolonged QT. The CDIATM Methadone Colloidal Gold Test Cassette (Urine) is a rapid urine-screening test that can be performed without the use of an instrument. The test utilizes a monoclonal antibody to selectively detect elevated levels of methadone in urine. The CDIATM Methadone Colloidal Gold Test Cassette (Urine) yields a positive result when the methadone in urine exceeds 300 ng/mL.