The CDIATM Methaqualone Colloidal Gold Test Cassette (Urine) is a lateral flow chromatographic immunoassay for the detection of methaqualone and its metabolites in human urine.
Methaqualone antigen is coated on the test region of the nitrocellulose membrane of the strips, and methaqualone antibody is labeled with colloid gold. During a test, the colloid gold labeled antibody coated in the strip move forward along the membrane, and a red line will show up when the antibody gathers with the antigen in the test line; if methaqualone in the sample is over the detection limit, line T is blank; on the other wise, if methaqualone in the sample is less than the detection limit, line T is red.
Methaqualone is a sedative-hypnotic drug that is similar in effect to barbiturates. The sedative-hypnotic activity was first noted by Indian researchers in the 1950s. Its use peaked in the early 1970s as a hypnotic, for the treatment of insomnia, and as a sedative and muscle relaxant. It has also been used illegally as a recreational drug. In 1965 a Methaqualone/antihistamine combination was sold as the sedative drug Mandrax, by Roussel Laboratories (now part of Sanofi-Aventis). Methaqualone is a depressant that increases the activity of the GABA receptors in the brain and nervous system. When GABA activity is increased, blood pressure drops and the breathing and pulse rates slow, leading to a state of deep relaxation. Methaqualone peaks in the bloodstream within several hours, its effects generally lasting four to eight hours. Regular users build up a physical tolerance, requiring larger doses for the same effect. Overdose can lead to nervous system shut down, coma and death. The CDIATM Methaqualone Colloidal Gold Test Cassette (Urine) is a rapid urine-screening test that can be performed without the use of an instrument. The test utilizes a monoclonal antibody to selectively detect elevated levels of methaqualone in urine. The CDIATM Methaqualone Colloidal Gold Test Cassette (Urine) yields a positive result when the methaqualone in urine exceeds 300 ng/mL.