The CDIATM Methylphenidate Colloidal Gold Test Cassette (Urine) is a lateral flow chromatographic immunoassay for the detection of methylphenidate and its metabolites in human urine.
Methylphenidate antigen is coated on the test region of the nitrocellulose membrane of the strips, and methylphenidate antibody is labeled with colloid gold. During a test, the colloid gold labeled antibody coated in the strip move forward along the membrane, and a red line will show up when the antibody gathers with the antigen in the test line; if methylphenidate in the sample is over the detection limit, line T is blank; on the other wise, if methylphenidate in the sample is less than the detection limit, line T is red.
Methylphenidate (Ritalin) is a psychostimulant drug approved for treatment of ADHD or attention-deficit hyperactivity disorder, postural orthostatic tachycardia syndrome and narcolepsy. Methylphenidate primarily acts as a norepinephrine-dopamine reuptake inhibitor. Methylphenidate is most active at modulating levels of dopamine and to a lesser extent norepinephrine. Similar to cocaine, methylphenidate binds to and blocks dopamine transporters and norepinephrine transporters. Methylphenidate has both dopamine transporter and norepinephrine transporter binding affinity, with the dextromethylphenidate enantiomers displaying a prominent affinity for the norepinephrine transporter. Methylphenidate may also exert a neuroprotective action against the neurotoxic effects of Parkinson's disease and methamphetamine abuse. Methylphenidate taken orally has a bioavailability of 11-52% with a duration of action around 1-4 hours for instant release, 3–8 hours for sustained release, and 8–12 hours for extended release (Concerta). The half-life of methylphenidate is 2 3 hours, depending on the individual. The peak plasma time is achieved at about 2 hours. The CDIATM Methylphenidate Colloidal Gold Test Cassette (Urine) is a rapid urine-screening test that can be performed without the use of an instrument. The test utilizes a monoclonal antibody to selectively detect elevated levels of methylphenidate in urine. The CDIATM Methylphenidate Colloidal Gold Test Cassette (Urine) yields a positive result when the methylphenidate in urine exceeds 300 ng/mL.