The CDIATM Myoglobin Immunofluorescence Test Cassette is designed for quantitative determination of myoglobin in human serum, whole blood or plasma.
The test uses an anti-human myoglobin monoclonal antibody conjugated with fluorescence latex and another anti-human myoglobin monoclonal antibody coated on the test line. After the sample has been applied to the test strip, the fluorescence latex-labelled anti-human myoglobin monoclonal antibody binds with the myoglobin in sample and forms a marked antigen-antibody complex. This complex moves to the test card detection zone by capillary action. Then marked antigen-antibody complex is captured on the test line by the anti-human myoglobin monoclonal antibody. The fluorescence intensity of the test line increases in proportion to the amount of myoglobin in sample. Then insert test card into the Fluorescent Immunoassay Analyzer FIA7100, the concentration of myoglobin in sample will be determined and displayed on the screen.
Myoglobin is the primary oxygen-carrying pigment of muscle, providing oxygen to the working muscles. When muscle is damaged, myoglobin is released into the bloodstream. Serum concentration of myoglobin increases as early as 1 hour after myocardial infarction, and peaks in approximately 4 to 8 hours after onset and generally falls back to normal within one day. Therefore, myoglobin is better suited for the early diagnosis of acute myocardial infarction (AMI). But because it is abundant in both cardiac and skeletal muscle, it lacks adequate specificity. Although a negative myoglobin result effectively rules out a heart attack, a positive result must be confirmed by testing for more specific troponin. This kit is intended to use in quantitative detection of myoglobin content in human serum, plasma or whole blood.