Cryptosporidiosis is a diarrhoeal disease caused by microscopic parasites of the genus Cryptosporidium. Once an animal or person is infected, the parasite lives in the intestine and passes in the stool. Both the disease and the parasite are commonly known as "Crypto." Giardiasis is a diarrhoeal illness seen throughout the world. It is caused by a flagellate protozoan parasite, Giardia intestinalis, also known as G. lamblia and G. duodenalis. The incidence of Giardia is generally higher in low-income countries (e.g. many countries of Africa, Asia, and South and Central America) where access to clean water and basic sanitation is lacking. In areas such as Western Europe and the United States of America, Giardia infection is associated with ingestion of contaminated water, person-to-person spread, recent foreign travel, and recreational swimming.
The Crypto-Giardia Rapid test is a qualitative lateral flow immunoassay for the detection of Crypto and Giardia antigens in human feces samples. The membrane is pre-coated with monoclonal antibodies against Cryptosporidium and Giardia antigens on the test line regions. During testing, the sample reacts with the particles coated with anti-Cryptosporidium antibodies and/or with anti-Giardia antibodies which were pre-dried on the test strip. The mixture moves upward on the membrane by capillary action. In the case of a positive result the specific antibodies present on the membrane will react with the mixture conjugates and generate one or two coloured lines. A green coloured line always appears in the control line and serves as verification that sufficient volume was added, that proper flow was obtained and as an internal control for the reagents.
Figure 1. Assay procedure (collected stool samples)
Figure 2. Interpretation of results
Reagents And Materials Provided
1. Card tests
2. Instructions for use