CDIA™ Human Salmonella Typhi Antibody Rapid Test - Creative Diagnostics

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CDIATM Human Salmonella typhi antibody Rapid test (DTSXYZ23)

Intended Use

The Salmonella typhi antibody Rapid is a rapid visual immunoassay for the qualitative presumptive detection of specific IgM and IgG antibodies to Salmonella typhi in human serum or plasma specimens. This kit is intended for use as an aid in the diagnosis of Salmonella typhi infection.
  • Serum, Plasma
  • Strip

Product Introduction

General Description

The Salmonella typhi Rapid Test detects IgM and IgG antibodies specific to Salmonella typhi through visual interpretation of color development on the internal strip. Salmonella typhi antigens are immobilized on the test region of the membrane. During testing, the specimen reacts with Salmonella typhi antigen conjugated to colored particles and precoated onto the conjugate pad of the test. The mixture then migrates through the membrane by capillary action, and interacts with reagents on the membrane. If there are sufficient antibodies to Salmonella typhi in the specimen, a colored band will form at the test region of the membrane. The presence of this colored band indicates a positive result, while its absence indicates a negative result. The appearance of a colored band at the control region serves as a procedural control, indicating that the proper volume of specimen has been added and membrane wicking has occurred.

Principle

Typhoid fever is a serious illness caused by a bacteria called Salmonella typhi. In the U.S. about 400 cases occur annually, and 70% of these are acquired while travelling internationally. Typhoid fever is still common in developing countries and affects about 12.5 million persons each year. Any person can get typhoid fever, but those who travel, especially to developing countries are at increased risk. Typhoid fever is most common in non-industrialized countries. Travelers to Asia, Africa, Eastern Europe, and Latin America are especially at risk. Salmonella typhi bacteria are shed in the urine or stool of infected persons, including chronic carriers. There are no known animal reservoirs for typhoid fever. Typhoid fever is spread by eating or drinking contaminated food or water or by direct or indirect contact with fecal material from infected persons. Symptoms of Salmonella typhi infection may be mild to severe and can include fever, headache, loss of appetite, constipation or diarrhea, and nonproductive cough. The symptoms may appear 3 days to 3 months(depending on the number of bacteria ingested), but onset of illness usually occurs 1-3 weeks after exposure. The time period that a person can have Salmonella typhi in their stool is variable. About 10% of untreated patients may shed infectious bacteria in their stool up to 3 months after onset of symptoms and 2%-5% may become permanent shedders. These permanent shedders are called chronic carriers.

Reagents And Materials Provided

1. Individually packed test devices: Each device contains a strip with colored conjugates and reactive reagents pre-spreaded at the corresponding regions. 

2. Buffer: 0.1 M Phosphate buffered saline (PBS) and 0.02% sodium azide. 

3. Disposable pipettes: For adding specimens 

4. Package insert: For operating instructions

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