* For research use only, not for use in diagnostic procedures.
The Vibrio cholerae O1 Rapid Test Device (Feces) detects Vibrio cholerae O1 through visual interpretation of color development on the internal strip. Anti- Vibrio cholerae O1 antibodies are immobilized on the test region of the membrane. During testing, the specimen reacts with anti- Vibrio cholerae O1 antibodies conjugated to colored particles and precoated onto the sample pad of the test. The mixture then migrates through the membrane by capillary action and interacts with reagents on the membrane. If there is sufficient Vibrio cholerae O1 in the specimen, a colored band will form at the test region of the membrane. The presence of this colored band indicates a positive result, while its absence indicates a negative result. The appearance of a colored band at the control region serves as a procedural control, indicating that the proper volume of specimen has been added and membrane wicking has occurred.
Cholera epidemics, caused by V.cholerae serotype O1, continue to be a devastating disease of immense global significance in many developing countries. Clinically, cholera may range from asymptomatic colonization to severe diarrhea with massive fluid loss, leading to dehydration, electrolyte disturbances, and death. V. cholerae O1 cause this secretory diarrhea by colonization of the small intestine and production of a potent cholera toxin, Because of the clinical and epidemiological importance of cholera, it is critical to determine as quickly as possible whether or not the organism from a patient with watery diarrhea is positive for V.cholera O1. A fast, simplr and reliable method for detecting V.cholerae O1 is a great value for clinicians in managing the disease and for public health officials in instituting control measures.
Reagents And Materials Provided
1. Individually packed test devices: Each device contains a strip with colored conjugates and reactive reagents pre-coated at the corresponding regions.
2. Specimens dilution tube with buffer: 0.1 M Phosphate buffered saline (PBS) and 0.02% sodium azide.
3. Disposable pipettes: For collecting of liquid specimens
4. Package insert: For operating instructions