CDIA™ Norovirus Test Kit - Creative Diagnostics

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CDIATM Norovirus Test Kit (DTS694)

Intended Use

One-step immunochromatographic test for the differential detection of Norovirus Genogroups I and II in human faeces. The Norovirus chromatographic immunoassay is a procedure for the qualitative detection, in separate bands, of Genogroup I (GI) and Genogroup II (GII) of Norovirus in human feces. A positive signal in either test band provides a good indication that we may be in presence of a Norovirus infection which should draw the attention of the clinician.
  • Stool
  • Kit
  • Detection limit for GI.1 = 25 ng/mlDetection limit for GII.4 = 10 ng/ml

Product Introduction

Principle Of The Test
The test is based on the immunological capture of coloured microbeads during its passage along a membrane on which specific monoclonal antibodies against GI and GII have been immobilized in two separate bands.
Norovirus test uses a combination of:
1) red latex particles conjugated to specific antibodies against GII which cooperates with another antibodies specific for GII located on the membrane under the control band.
2) other red latex particles conjugated to specific antibodies against GI which cooperates with another antibodies specific for GI located on the membrane above the control band.
3) blue latex particles conjugated to an antigen recognized by an antibody specific for this antigen bound to the membrane forming the so called test control band. In this test the sample is treated first with the sample diluent buffer (included in the kit) to achieve the extraction of the virus from the stool matrix. After the extraction, you just need to add a certain volume of supernatant onto the test strip and wait for 15 minutes. When the extracted sample flows through the test membrane, the coloured particles migrate. In the case of a positive sample, specific antibodies on the membrane will capture the coloured particles covered by the antigen. Depending on the virus content of the sample, different coloured lines will be visible. These lines are used for interpretation of results, following a 15-minute incubation at room temperature.

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