The CDIATM NT-proBNP Immunofluorescence Test Cassette is designed for quantitative determination of NT-proBNP in human serum, whole blood or plasma.
The test uses an anti-human NT-proBNP monoclonal antibody conjugated with fluorescence latex and another anti-human NT-proBNP monoclonal antibody coated on the test line. After the sample has been applied to the test strip, the fluorescence latex-labelled anti-human NT-proBNP monoclonal antibody binds with the NT-proBNP in sample and forms a marked antigen-antibody complex. This complex moves to the test card detection zone by capillary action. Then marked antigen-antibody complex is captured on the test line by the anti-human NT-proBNP monoclonal antibody. The fluorescence intensity of the test line increases in proportion to the amount of NT-proBNP in sample. Then insert test card into the Fluorescent Immunoassay Analyzer FIA7100, the concentration of NT-proBNP in sample will be determined and displayed on the screen.
The N-terminal prohormone of brain natriuretic peptide (NT-proBNP or BNPT) is a prohormone with a 76 amino acid N-terminal inactive protein that is cleaved from the molecule to release brain natriuretic peptide.Both BNP and NT-proBNP levels in the blood are used for screening, diagnosis of acute congestive heart failure (CHF) and may be useful to establish prognosis in heart failure, as both markers are typically higher in patients with worse outcome.The plasma concentrations of both BNP and NT-proBNP are also typically increased in patients with asymptomatic or symptomatic left ventricular dysfunction and is associated with coronary artery disease and myocardial ischemia. This kit is intended to use in quantitative detection of NT-proBNP content in human serum, plasma or whole blood.