CDIA™ Potato Virus S (PVS) Test Cassette - Creative Diagnostics

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Plant Pathogen Tests | PVS |

CDIATM Potato Virus S (PVS) Test Cassette (DTSPV8)

* For research use only, not for use in diagnostic procedures.

Intended Use

The test can be simply used to on-site and rapid detect PVS in suspicious plant samples, without requiring any special equipment or expertise to run.
  • suspicious plant samples
  • Cassette
  • Sensitivity was comparable to the DAS-ELISA format and strongest coloration of testbands was obtained with a 1:60 to 1:120 (w/v) dilution of an extrac

Product Introduction

General Description

Potato virus S (PVS) is of increasing importance in potato. PVS can cause yield loss up to 20%. Most potato cultivars are symptomless, so it is very difficult to detect using visual cues. PVS is non-persistently transmitted by aphids.

Principle Of The Test

The development of rapid test strips is based on Lateral Flow Device (LFD) technology using specific antibodies. A LFD format consists of antibody-coated latex beads latex (or colloidal gold) which will bind the specific pathogen antigen absorbed from the plant extract. The agglutination is accumulated at a specific location by the presence of a line of target specific antibody, which immobilises the agglutinated latex whilst allowing the background reagents to be washed away by continued flow along the membrane. The membrane contains a strip of target-specific antibody, test strip and a strip of another antibody that binds to the specific antibody, control strip. Latex beads containing bound antigen are trapped on the test strip leaving a visible line. Surplus latex beads that do not contain antigen are trapped on the control strip showing that the assay has worked. This provides a non-subjective and clear read out of a positive result against a low background.

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