Although a wide variety of viral agents are capable of causing lower respiratory tract infections in children and adults, Influenza A & B; respiratory syncytial virus (RSV); parainfluenza viruses 1, 2, and 3; and Adenovirus are the most common. Of these, Influenza A & B and RSV are the most important causes of medically attended acute respiratory illness. In addition to sharing a similar seasonal prevalence, it is important to remain cognizant that Influenza A & B and RSV share overlapping clinical features and infection potential for certain high-risk patient groups (e.g. extremes of age, underlying cardiopulmonary disease and immunosuppression). Symptoms of respiratory illness caused by adenovirus infection range from the common cold syndrome to pneumonia, croup, and bronchitis.
The RSV-Adenovirus Resp. Rapid test is a qualitative lateral flow immunoassay for the detection of RSV and Adenovirus Respiratory antigen in human nasopharyngeal samples. The membrane is pre-coated with monoclonal antibodies against RSV and Adenovirus antigens on the test line regions. During testing, the sample reacts with the particles coated with anti-RSV antibodies and/or anti-Adenovirus antibodies which were pre-dried on the test strip. The mixture moves upward on the membrane by capillary action. In the case of a positive result the specific antibodies present on the membrane will react with the mixture conjugate and generate one or two coloured lines. A green coloured line always appears in the control line and serves as verification that sufficient volume was added, that proper flow was obtained and as an internal control for the reagents.
Figure 1. Assay procedure (collected nasopharyngeal swab)
Figure 2. Assay procedure (nasal wash or aspirate samples)
Figure 3. Interpretation of results
Reagents And Materials Provided
1. Blister tests
2. Instructions for use
3. Diluent (Sample diluent) 2
5. Testing tubes or vials
6. Plastic pipettes
7. RSV and Adenovirus Positive Control swabs