The Sulfamethazine Residue Rapid Test Device is used to qualitative detection of Sulfamethazine in honey samples at the sensitivity of 10
Sulfamethazine (SM2) is a broad spectrum antibiotic which is widely used as bacteriostatic agents in animal husbandry and veterinary practice. Combined with inhibitors of dihydrofolate reductase such as trimethoprim, tetroxoprim, or pyrimethamine Sulfamethazine are also used in veterinary medicine for the treatment of intestinal infections, mastitis, pulmonitis and other (systemic) diseases. However, it leads side effects of hemato-toxic, agranulocytosis, hypersensitiveness . It will affect urinary system and cranial nerves system. Therefore, it is possible that Sulfadiazine residues, after use in illegal practice, may lead to a risk for consumers.
PRINCIPLE OF THE TEST
Competitive assays are primarily used for testing small mole-cules. If Sulfamethazine is present in the sample it will therefore bind with the conjugate and will be labelled. As the sample mi-grates along the membrane and reaches the capture zone an excess of labelled antibody will bind to the immobilised antigen so that no visible line is produced. The bound conjugate will then bind to the antibodies in the control zone producing a visible control line. A single control line on the membrane is a positive result. Two visible lines in the capture and control zones is a negative result. However, if an excess of unlabelled Sul-famethazine is not present, a weak line may be produced in the capture zone, indicating an inconclusive result.