The CDIATM TCA Colloidal Gold Test Cassette (Urine) is a lateral flow chromatographic immunoassay for the detection of TCA and its metabolites in human urine.
Tricyclic antidepressants antigen is coated on the test region of the nitrocellulose membrane of the strips, and tricyclic antidepressants antibody is labeled with colloid gold. During a test, the colloid gold labeled antibody coated in the strip move forward along the membrane, and a red line will show up when the antibody gathers with the antigen in the test line; if tricyclic antidepressants in the sample is over the detection limit, line T is blank; on the other wise, if tricyclic antidepressants in the sample is less than the detection limit, line T is red.
Tricyclic Antidepressants are a group of antidepressant drugs that are commonly used for treatment of depressive disorders. TCAs can be taken orally or by intramuscularly injection (IM). The symptoms of TCAs overdoses include agitation, confusion, hallucinations, hypertonicity, seizures, and EKG changes. The half-life of TCA varies from a few hours to several days. The commonly used TCAs are excreted with a very low percentage of unchanged drugs in the urine. Therefore, detection of the metabolites of TCAs in human urine has been used for screening the abuse of TCAs. The CDIATM TCA Colloidal Gold Test Cassette (Urine) is a rapid urine-screening test that can be performed without the use of an instrument. The test utilizes a monoclonal antibody to selectively detect elevated levels of TCA in urine. The CDIATM TCA Colloidal Gold Test Cassette (Urine) yields a positive result when the TCA in urine exceeds 1000 ng/mL.