TMV is an extremely stable virus that causes diseases in a very wide host range which includes at least 199 species from 30 plant families. It is normally transmitted mechanically or sometimes by chewing insects. In tobacco the virus elicits a mosaic but may also cause veinbanding. One of the common control methods for TMV is sanitation, which includes removing infected plants, and washing hands in between each planting.
Principle Of The Test
The development of rapid test strips is based on Lateral Flow Device (LFD) technology using specific antibodies. A LFD format consists of antibody-coated latex beads latex (or colloidal gold) which will bind the specific pathogen antigen absorbed from the plant extract. The agglutination is accumulated at a specific location by the presence of a line of target specific antibody, which immobilises the agglutinated latex whilst allowing the background reagents to be washed away by continued flow along the membrane. The membrane contains a strip of target-specific antibody, test strip and a strip of another antibody that binds to the specific antibody, control strip. Latex beads containing bound antigen are trapped on the test strip leaving a visible line. Surplus latex beads that do not contain antigen are trapped on the control strip showing that the assay has worked. This provides a non-subjective and clear read out of a positive result against a low background.
Reagents And Materials Provided
1. 50 single-use test cassettes
2. 50 pre-filled extraction tubes with sample buffer
3. 50 disposable pipettes for sample application