This kit for Cry1 Ab/Ac is intended to qualitatively determine the presence of the Cry1 Ab/Ac Bt endotoxins in genetically modified organisms from crops such as cotton or corn, etc.
Based on the principle of double antibody sandwich immunochromatography, the antigen in the sample in the lateral movement of the colloidal gold-labeled specific monoclonal antibody binding to form antigen-antibody complex, continue to the front flow and NC membrane test region specific anti monoclonal antibody binds to form a double antibody sandwich complex. The result is the formation of a colored band in the test region. If there is no BT Cry1 in the sample, the area will remain colorless. The sample continues to move to the control area and forms a pink to purple color, indicating the test is working and the result is valid.
BT Cry1 is a gene found in Bacillus thuringiensis, a gram-positive bacterium that produces a toxin that can be utilized as a pesticide. The gene BT Cry1 encodes the protein that is toxic to insects. When expressed and produced in crops such as cotton, corn, it acts as a built-in insecticide for the plants. Due to the protein’s toxic nature, there are concerns that this gene will have a negative impact on the consumer’s health.
CDIATM Transgene Cry1 Ab/Ac colloidal gold test for Cry1 Ab/Ac is designed to extract and detect the presence of the Cry1 Ab/Ac Bt endotoxins at the levels typically expressed in genetically modified crop plant tissue.